Dubos Oleic HiVeg Broth Base with Antibiotic and Oleic Albumin Supplement

[所属分类:培养基配方] [发布时间:2020-6-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dubos Oleic HiVeg Broth Base
with Antibiotic and Oleic Albumin Supplement

培养基配方
Composition per liter:
Na2HPO4 ...................................................................2.5g
Asparagine.................................................................1.0g
KH2PO4 ....................................................................1.0g
Plant hydrolysate..........................................................0.5g
Ferric ammonium citrate...................................................0.05g
MgSO4 ....................................................................0.01g
CaCl2 ....................................................................0.5mg
CuSO4 ....................................................................0.1mg
ZnSO4 ....................................................................0.1mg
Penicllin solution.......................................................10.0mL
pH 6.5 ± 0.2 at 25°C
Source: This medium, without penicillin and oleic albumin supple-
ment, is available as a premixed powder from HiMedia.
Penicillin Solution:
Composition per 10.0mL:
Penicillin.............................................................10,000U
Preparation of Penicllin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Oleic Albumin Supplement:
Composition per 100.0mL:
Oleic acid................................................................10.0g
Albumin fraction IV .......................................................4.0g
NaCl......................................................................0.68g
NaOH......................................................................8.0mg
Preparation of Oleic Albumin Supplement: Add components
to distilled/deionized water and bring volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except penicllin solu-
tion and oleic albumin supplement, to distilled/deionized water and
bring volume to 880.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 10.0mL sterile penicillin solution and 100.0mL
sterile oleic albumin solution. Mix thoroughly. Aseptically distribute
into sterile tubes.
Use: For the cultivation of Mycobacterium tuberculosis and other
Mycobacterium species.