Esculin Mannitol Agar

[所属分类:培养基配方] [发布时间:2020-7-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Esculin Mannitol Agar

培养基配方
Composition per liter:
Agar..........................................................................13.5g
Polypeptone™ .................................................................10.0g
D- Mannitol ..................................................................10.0g
Pancreatic digest of casein....................................................5.0g
Yeast extract..................................................................5.0g
NaCl...........................................................................5.0g
Heart peptone..................................................................3.0g
Cornstarch.....................................................................1.0g
Esculin........................................................................1.0g
Ferric ammonium citrate........................................................0.5g
Phenol Red...................................................................0.025g
Nalidixic acid solution......................................................10.0mL
Colistin solution............................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Nalidixic Acid Solution:
Composition per 10.0mL:
Nalidixic acid...............................................................0.015g
Preparation of Nalidixic Acid Solution: Add nalidixic acid to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Colistin Solution:
Composition per 10.0mL:
Colistin......................................................................0.01g
Preparation of Colistin Solution: Add colistin to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except nalidixic acid
solution and colistin solution, to distilled/deionized water and bring
volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile nalidixic acid solution and colistin solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the selective isolation, cultivation, and differentiation of
Staphylococcus aureus and group D streptococci based on mannitol
fermentation and hydrolysis of esculin. Bacteria that ferment mannitol
appear as yellow colonies surrounded by a yellow zone. Bacteria that
hydrolyze esculin appear as dark brown to black colonies surrounded
by a dark brown to black zone.