Esculin Thallium Medium

[所属分类:培养基配方] [发布时间:2020-7-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Esculin Thallium Medium

培养基配方
Composition per liter:
Agar............................................................................15.0g
Beef extract....................................................................10.0g
Peptone.........................................................................10.0g
NaCl.............................................................................5.0g
Esculin .........................................................................1.0g
Thallous sulfate................................................................0.33g
Crystal Violet..................................................................1.3mg
Blood, bovine or sheep ........................................................50.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except blood, to dis-
tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add sterile blood. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Caution: Thallium salts are toxic.
Use: For the cultivation of Streptococcus species that cause bovine
mastitis.