Eugon Agar with Fildes Enrichment (LMG Medium 75)

[所属分类:培养基配方] [发布时间:2020-7-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Eugon Agar with Fildes Enrichment
(LMG Medium 75)

培养基配方
Composition per liter:
Agar.......................................................................15.0g
Pancreatic digest of casein................................................15.0g
Glucose.....................................................................5.5g
Papaic digest of soybean meal...............................................5.0g
NaCl........................................................................4.0g
L- Cystine..................................................................0.7g
Na2SO3 .....................................................................0.2g
Fildes digested sheep blood...............................................50.0mL
pH 7.0 ± 0.2 at 25°C
Fildes Enrichment Solution:
Composition per 206.0mL:
Pepsin......................................................................1.0g
NaCl (0.85% solution)....................................................150.0mL
Sheep blood, defibrinated.................................................50.0mL
HCl........................................................................6.0mL
pH 7.0–7.2 at 25°C
Source:  Fildes enrichment solution is available from BD Diagnostic
Systems.
Preparation of Fildes Enrichment Solution: Combine compo-
nents. Mix thoroughly. Incubate at 56°C for 4 hr. Bring pH to 7.0 with
20% NaOH. Adjust pH to 7.2 with HCl. Do not autoclave. Add 0.25
mL of chloroform and store at 4°C. Before use, heat to 56°C to remove
chloroform.
Preparation of Medium: Add components, except Fildes digested
sheep blood, to 950.0mL distilled/deionized water. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°fl55°C. Aseptically add 50.0mL sterile Fildes
digested sheep blood. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use:  For the cultivation of Taylorella equigenitalis.