Eugon Agar with Fildes Enrichment

[所属分类:培养基配方] [发布时间:2020-7-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Eugon Agar with Fildes Enrichment

培养基配方
Composition per liter:
Agar.....................................................................15.0g
Pancreatic digest of casein..............................................15.0g
Glucose...................................................................5.5g
Papaic digest of soybean meal.............................................5.0g
NaCl......................................................................4.0g
L- Cystine................................................................0.3g
Na2SO3 ...................................................................0.2g
Fildes enrichment solution..............................................50.0mL
pH 7.0 ± 2.0 at 25°C
Fildes Enrichment Solution:
Composition per 206.0mL:
Pepsin...................................................................1.0g
NaCl (0.85% solution).................................................150.0mL
Sheep blood, defibrinated..............................................50.0mL
HCl.....................................................................6.0mL
pH 7.0–7.2 at 25°C
Source:  Fildes enrichment solution is available from BD Diagnostic
Systems.
Preparation of Fildes Enrichment Solution: Combine compo-
nents. Mix thoroughly. Incubate at 56°C for 4 hr. Bring pH to 7.0 with
20% NaOH. Adjust pH to 7.2 with HCl. Do not autoclave. Add 0.25
mL of chloroform and store at 4°C. Before use, heat to 56°C to remove
chloroform.
Preparation of Medium: Add components, except Fildes enrich-
ment solution, to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 13 psi pressure–118°C. Cool to 50°–55°C. Aseptically add 50.0mL
of Fildes enrichment solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Taylorella equigenitalis.