Eugon Blood Agar (Eugonagar™)

[所属分类:培养基配方] [发布时间:2020-7-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Eugon Blood Agar
(Eugonagar™)

培养基配方
Composition per liter:
Agar ........................................................................15.0g
Pancreatic digest of casein..................................................15.0g
Glucose ......................................................................5.5g
Papaic digest of soybean meal.................................................5.0g
NaCl..........................................................................4.0g
L- Cystine ...................................................................0.3g
Na2SO3 .......................................................................0.2g
Sheep blood, defibrinated..................................................100.0mL
pH 7.0 ± 2.0 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 900.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 13 psi pressure–
118°C. Cool to 45°–50°C. Aseptically add sterile sheep blood. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes. If de-
sired, medium may be chocolatized by maintaining at 80°–85°C for 20
min after the addition of sheep blood.
Use: For the cultivation and maintenance of fastidious microorgan-
isms. For the cultivation and maintenance of Bifidobacterium species.