Fastidious Anaerobe Agar, Selective with Neomycin and Vancomycin (FAA Selective with Neomycin and Vancomycin)

[所属分类:培养基配方] [发布时间:2020-7-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fastidious Anaerobe Agar, Selective
with Neomycin and Vancomycin
(FAA Selective with Neomycin and Vancomycin)

培养基配方
Composition per liter:
Peptone ...................................................................23.0g
Agar.......................................................................12.0g
NaCl........................................................................5.0g
Glucose.....................................................................1.0g
L- Arginine.................................................................1.0g
Sodium pyruvate.............................................................1.0g
Soluble starch..............................................................1.0g
L -Cysteine·HCl·H2O.......................................................0.5g
Sodium succinate............................................................0.5g
NaHCO3 .....................................................................0.4g
Na4P2O7 ·10H2O............................................................0.25g
Neomycin....................................................................0.1g
Sheep blood, defibrinated.................................................50.0mL
Vancomycin solution ......................................................10.0mL
Hemin solution.............................................................1.0mL
Vitamin K 1  solution......................................................0.1mL
pH 7.2 ± 0.2 at 25°C
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ................................................................1.0g
Ethanol...................................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Hemin Solution:
Composition per 100.0mL:
Hemin .....................................................................1.0g
NaOH (1N solution).......................................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Vancomycin Solution:
Composition per 10.0mL:
Vancomycin...............................................................7.5mg
Preparation of Vancomycin Solution: Add vancomycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except defibrinated
sheep blood and vancomycin solution, to distilled/deionized water and
bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 50.0mL of sterile defibrinated sheep blood and
10.0mL of vancomycin solution. Mix thoroughly. Pour into sterile Pe-
tri dishes or distribute into sterile tubes.
Use: For the selective cultivation of Fusobacterium species from clin-
ical and nonclinical specimens.