Fay and Barry Medium

[所属分类:培养基配方] [发布时间:2020-7-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fay and Barry Medium

培养基配方
Composition per liter:
Amino acid.................................................................10.0g
Peptone.....................................................................5.0g
Yeast extract...............................................................3.0g
Bromcresol Purple solution ................................................5.0mL
pH 5.5 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 100.0mL:
Bromcresol Purple .........................................................0.2g
Ethanol..................................................................50.0mL
Preparation of Bromcresol Purple Solution:  Add Bromcresol
Purple to 50.0mL of absolute ethanol. Add distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. The amino acid may be  L- arginine,  L-
ornithine, or  L- lysine, depending on which amino acid decarboxylase
activity is being measured. Mix thoroughly. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the determination of decarboxylase activities of Aeromonas
species.