Fermentation HiVeg Medium for Neisseriae with Carbohydrate

[所属分类:培养基配方] [发布时间:2020-7-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fermentation HiVeg Medium for Neisseriae
with Carbohydrate

培养基配方
Composition per liter:
Plant hydrolysate ..........................................................20.0g
NaCl.........................................................................5.0g
Agar.........................................................................3.5g
Cystine......................................................................0.5g
Na2SO3 ......................................................................0.5g
Phenol Red.................................................................0.017g
Carbohydrate solution.....................................................100.0mL
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Adonitol, ara-
binose, cellobiose, glucose, dulcitol, fructose, galactose, inositol, lac-
tose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,
trehalose, xylose, or other carbohydrates may be used. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 12 psi pressure–118°C. Cool to 50°C.
Aseptically add 100.0mL of sterile carbohydrate solution. Mix thor-
oughly.
Use: For the cultivation and identification of Neisseria spp. For study-
ing fermentation reactions of fastidious organisms such as Neisseria
species.