Fermentation HiVeg Medium Base for C. perfringens with Salicin and Raffinose

[所属分类:培养基配方] [发布时间:2020-7-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fermentation HiVeg Medium Base for C. perfringens
with Salicin and Raffinose

培养基配方
Composition per liter:
Plant hydrolysate ......................................................10.0g
Plant special peptone ..................................................10.0g
Agar.....................................................................2.0g
Na-thioglycollate ......................................................0.25g
Salicin solution .......................................................10.0mL
Raffinose solution......................................................10.0mL
pH 7.0 ± 2.0 at 25°C
Source: This medium, without salicin and raffinose, is available as a
premixed powder from HiMedia.
Salicin Solution:
Composition per 10.0mL:
Salicin...................................................................0.1g
Preparation of Salicin Solution: Add salicin to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Raffinose Solution:
Composition per 10.0mL:
Raffinose.................................................................0.1g
Preparation of Raffinose Solution: Add raffinose to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except salicin and
raffinose solutions, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
10.0mL sterile salicin solution and 10.0mL sterile raffinose solution.
Mix thoroughly. Aseptically distribute into tubes or flasks.
Use:  For the cultivation of Clostridium perfringens.