Fermentation Broth (CHO Medium)

[所属分类:培养基配方] [发布时间:2020-7-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fermentation Broth
(CHO Medium)

培养基配方
Composition per liter:
Pancreatic digest of casein.................................................15.0g
Yeast extract................................................................7.0g
NaCl.........................................................................2.5g
Agar .......................................................................0.75g
Sodium thioglycolate.........................................................0.5g
L- Cystine .................................................................0.25g
Ascorbic acid ...............................................................0.1g
Bromthymol Blue ............................................................0.01g
Carbohydrate or starch solution...........................................100.0mL
pH 7.0 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate..............................................................6.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Starch Solution:
Composition per 100.0mL:
Starch .....................................................................2.5g
Preparation of Starch Solution: Add starch to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL
of sterile carbohydrate solution. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks. Loosen caps on tubes. Place in an anaerobic
chamber under an atmosphere of 85% N2 , 10% H2 , and 5% CO2 . Fas-
ten the caps securely or maintain in an anaerobic chamber.
Use: For the differentiation of anaerobic bacteria based upon carbohy-
drate fermentation. Bacteria that ferment carbohydrates turn the
medium yellow.