Fermentation Basal Medium

[所属分类:培养基配方] [发布时间:2020-7-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fermentation Basal Medium

培养基配方
Composition per liter:
Agar.......................................................................15.0g
(NH4)2HPO4 .................................................................1.0g
MgSO4 ·7H2O................................................................0.2g
KCl........................................................................0.02g
Carbohydrate solution....................................................100.0mL
Bromcresol Purple solution ...............................................20.0mL
pH 7.0 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate..............................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Bromcresol Purple Solution:
Composition per 100.0mL:
Bromcresol Purple .........................................................0.04g
Ethanol....................................................................50.0mL
Preparation of Bromcresol Purple Solution:  Add Bromcresol
Purple to 50.0mL of absolute ethanol. Add distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL
of sterile carbohydrate solution. Various carbohydrates are used for dif-
ferent fermentation tests. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the differentiation of aerobic actinomycetes based upon car-
bohydrate fermentation. Actinomycetes that produce acid from carbo-
hydrates turn the medium yellow.