Ferroplasma acidiphilum Medium (DSMZ Medium 874)

[所属分类:培养基配方] [发布时间:2020-7-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ferroplasma acidiphilum Medium
(DSMZ Medium 874)

培养基配方
Composition per 1001.6mL:
Solution A................................................................950.0mL
Solution B.................................................................10.0mL
Solution C.................................................................1.6mL
pH 1.7 ± 0.2 at 25°C
Solution A:
Composition per 950.0mL:
MgSO4 ·7H2O................................................................0.4g
(NH4)2SO4 ..................................................................0.2g
KCl.........................................................................0.1g
K2HPO4 .....................................................................0.1g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 950.0mL. Mix thoroughly.
Solution B:
Composition per 50.0mL:
FeSO4 ·7H2O..............................................................25.0g
H2SO4 , 1N...............................................................40.0mL
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 50.0mL. Mix thoroughly.
Solution C:
Composition per 10.0mL:
Yeast extract............................................................1.0g
Preparation of Solution C:  Add yeast extract to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add 50.0mL solution B to 950.0mL so-
lution A. Mix thoroughly. Adjust pH to 1.6–1.8 with H2SO4 . Filter ster-
ilize. Aseptically add 1.6 mL of sterile solution C. Pour into sterile
Petri dishes or leave in tubes. Mix thoroughly. Aseptically distribute to
sterile tubes or flasks.
Use: For the cultivation of Ferroplasma acidiphilum (Ferriplasma aci-
dophilum).