Ferrous Sulfate/Yeast Extract Medium (DSMZ Medium 1190)

[所属分类:培养基配方] [发布时间:2020-7-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ferrous Sulfate/Yeast Extract Medium
(DSMZ Medium 1190)

培养基配方
Composition per liter:
Yeast extract...........................................................0.2g
Basal salts solution .................................................20.0mL
Ferrous sulfate solution..............................................20.0mL
Trace elements solution................................................1.0mL
Basal Salts Solution:
Composition per liter :
MgSO4 ·7H2O............................................................25.0g
(NH4)2SO4 ..............................................................22.5g
Na2SO4 ·10H2O ..........................................................7.5g
KCl......................................................................2.5g
KH2PO4 ..................................................................2.5g
Ca(NO3)·4H2O............................................................0.7g
Preparation of Basal Salts Solution:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution:
Composition per liter :
ZnSO4 ·7H2O..............................................................10.0g
CuSO4 ·5H2O...............................................................1.0g
CoO4 ·7H2O................................................................1.0g
MnSO4 ·2H2O...............................................................1.0g
NiSO4 ·6H2O...............................................................1.0g
Na2SeO4 ...................................................................1.0g
Na2WO4 ·2H2O..............................................................1.0g
Cr2(SO4)3 ·15H2O..........................................................0.5g
H3BO3 .....................................................................0.6g
Na2MoO4 ·2H2O.............................................................0.5g
NaVO3 .....................................................................0.1g
Preparation of Trace Elements Solution: Adjust pH of 800.0mL
of distilled/deionized water to 2.0 with dilute H2SO4 . Add the above
salts in order one at a time, allowing each to dissolve before adding the
next. Maintain the pH at 2.0 by adding H2SO4  as necessary. After ad-
dition of vanadate, bring volume to 1.0L with water. Adjust final pH to
2.0. Autoclave for 15 min at 15 psi pressure–121°C. The vanadate will
require several days to dissolve.
Ferrous Sulfate Solution:
Composition per 20.0mL:
FeSO4 ·7H2O.............................................................2.78g
Preparation of Ferrous Sulfate Solution:  Adjust pH of 20.0mL
of distilled/deionized water to 1.8 with H2SO4 . Add FeSO4 ·7H2O. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except ferrous sulfate
solution, to distilled/deionized water and bring volume to 980.0mL.
Mix thoroughly. Adjust pH to 2.0 with H2SO4 . Gently heat while stir-
ring and bring to boiling. Boil for 1 min. Autoclave for 20 min at 15 psi
pressure–121°C. Cool to room temperature. Aseptically add the fer-
rous sulfate solution.
Use: For the cultivation of Sulfobacillus spp.