F-G Agar (Feeley-Gorman Agar)

[所属分类:培养基配方] [发布时间:2020-7-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

F-G Agar
(Feeley-Gorman Agar)

培养基配方
Composition per liter:
Casein, acid hydrolyzed.................................................17.5g
Agar ...................................................................17.0g
Beef extract.............................................................3.0g
Starch ..................................................................1.5g
L -Cysteine solution...................................................10.0mL
Fe4(P2O7)3 solution....................................................10.0mL
pH 6.9 ± 0.05 at 25°C
L -Cysteine Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H2O....................................................0.4g
Preparation of  L -Cysteine Solution: Add  L- cysteine·HCl·H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Fe4(P2O7)3 Solution:
Composition per 10.0mL:
Fe4(P2O7)3 ...............................................................0.25g
Preparation of Fe4 (P2O7)3 Solution: Add Fe4(P2O7)3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except  L -cysteine solu-
tion and Fe4(P2O7)3 solution, to distilled/deionized water and bring
volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of  L -cysteine solution. Mix thoroughly. Asep-
tically add 10.0mL of Fe4(P2O7)3 solution. Mix thoroughly. Adjust pH
to 6.9. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the isolation and cultivation of Legionella pneumophila.