​F-G Agar with Selenium (Feeley-Gorman Agar with Selenium)

[所属分类:培养基配方] [发布时间:2020-7-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

F-G Agar with Selenium
(Feeley-Gorman Agar with Selenium)

培养基配方
Composition per liter:
Casein, acid hydrolyzed....................................................17.5g
Agar ......................................................................17.0g
Beef extract...............................................................3.0g
Starch ....................................................................1.5g
L -Cysteine solution.......................................................10.0mL
Fe4(P2O7)3 solution........................................................10.0mL
Na2SeO3 ·5H2O solution....................................................10.0mL
pH 6.9 ± 0.05 at 25°C
L -Cysteine Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H2O........................................................0.4g
Preparation of  L -Cysteine Solution: Add  L- cysteine·HCl·H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Fe4(P2O7)3 Solution:
Composition per 10.0mL:
Fe4(P2O7)3 .................................................................0.25g
Preparation of Fe4 (P2O7)3 Solution: Add Fe4(P2O7)3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Na2SeO3 ·5H2O Solution:
Composition per 10.0mL:
Na2SeO3 ·5H2O...............................................................0.01g
Preparation of Na2SeO3 ·5H2O Solution: Add Na2SeO3 ·5H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components—except  L -cysteine so-
lution, Fe4(P2O7)3 solution, and Na2SeO3 ·5H2O solution—to distilled/
deionized water and bring volume to 970.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile  L -cysteine
solution. Mix thoroughly. Aseptically add 10.0mL of sterile Fe4(P2O7)3
solution and 10.0mL of sterile Na2SeO3 ·5H2O solution. Mix thorough-
ly. Adjust pH to 6.9. Pour into sterile Petri dishes or distribute into ster-
ile tubes.
Use:  For the isolation and cultivation of Legionella pneumophila.