Fletcher Medium

[所属分类:培养基配方] [发布时间:2020-7-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fletcher Medium

培养基配方
Composition per liter:
Agar ..........................................................................1.5g
NaCl...........................................................................0.5g
Peptone........................................................................0.3g
Beef extract...................................................................0.2g
Rabbit serum.................................................................50.0mL
pH 7.9 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD
Diagnostic Systems.
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of sterile
rabbit serum. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use: For the isolation, cultivation, and maintenance of cultures of Lep-
tospira species.