​Fletcher Medium with Fluorouracil (Fluorouracil Leptospira Medium)

[所属分类:培养基配方] [发布时间:2020-7-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fletcher Medium with Fluorouracil
(Fluorouracil Leptospira Medium)

培养基配方
Composition per liter:
Agar.............................................................................1.5g
NaCl.............................................................................0.5g
Peptone .........................................................................0.3g
Beef extract.....................................................................0.2g
Rabbit serum...................................................................50.0mL
Fluorouracil solution..........................................................20.0mL
pH 7.9 ± 0.1 at 25°C
Fluorouracil Solution:
Composition per 100.0mL:
Fluorouracil...................................................................10.0g
Preparation of Fluorouracil Solution: Add fluorouracil to
50.0mL of distilled/deionized water. Add 1.0mL of 2N NaOH and
bring volume to 100.0mL. Gently heat to 56°C for 2 hr. Adjust pH to
7.4–7.6 with NaOH. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except rabbit serum
and fluorouracil solution, to distilled/deionized water and bring vol-
ume to 930.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 80.0mL of sterile rabbit serum. Mix thoroughly. Asep-
tically distribute into sterile tubes or flasks. Immediately prior to use,
add 0.1mL of fluorouracil solution per 5.0mL of medium.
Use: For the isolation, cultivation, and maintenance of cultures of Lep-
tospira species.