FM Medium

[所属分类:培养基配方] [发布时间:2021-3-2] [发布人: ] [阅读次数:] [返回]

FM Medium

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
Pancreatic digest of casein..........................................................15.0g
Glucose .........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
L- Cysteine·HCl·H 2 O....................................................................0.75g
Crystal Violet..............................................................................0.01g
Horse serum.............................................................................50.0mL
Streptomycin solution..............................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Streptomycin Solution:
Composition per 10.0mL:
Streptomycin...............................................................................0.01g
Preparation of Streptomycin Solution: Add streptomycin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except horse serum and streptomycin solution, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse serum and streptomycin solution. Mixthoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation and cultivation of Fusobacterium species.