- 定做培养基/定制培养基
- 2020年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
FN Medium
FN MediumFN Medium
(Fluorescence Denitrification Medium)
培养基配方
Composition per liter:
Agar ............................................................................................15.0g
Proteose peptone No. 3...............................................................10.0g
KNO 3 ............................................................................................2.0g
K 2 HPO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................1.5g
NaNO 2 ...........................................................................................0.5g
pH 7.2 ±0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the differentiation of pseudomonads from other nonfermentative bacilli. Denitrification from nitrate or nitrite is indicated by the formation of gas bubbles in the solid medium. Pseudomonas aeruginosa, Pseudomonas mendocina, and Pseudomonas denitrificans are positive for denitrification. Fluorescein production is indicated by fluorescence under UV light. Pseudomonas aeruginosa is positive for fluorescein production; Pseudomonas denitrificans does not produce fluorescein.