FNA Medium

[所属分类:培养基配方] [发布时间:2021-3-2] [发布人: ] [阅读次数:] [返回]

FNA Medium
(Fluorescein Denitrification Agar)

培养基配方

Composition per liter:
Agar............................................................................................15.0g
Peptic digest of animal tissue .......................................................5.0g
Casein enzymatic hydrolysate ......................................................5.0g
KNO 3 ............................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................1.5g
K 2 HPO 4 .........................................................................................1.5g
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the differentiation of Pseudomonas spp. based upon their ability to reduce nitrates or nitrites to nitrogen gas and the detection of fluorescein pigment production.