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Folic Acid Agar

[所属分类:培养基配方] [发布时间:2021-3-2] [发布人: ] [阅读次数:] [返回]

Folic Acid Agar

培养基配方

Composition per liter:
Noble agar...................................................................................15.0g
K 2 HPO 4 ·3H 2 O ..............................................................................1.2g
Folic acid ......................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
Salts A........................................................................................5.0mL
Salts B........................................................................................1.5mL
Salts A:
Composition per 100.0mL:
MgSO 4 ·7H 2 O................................................................................1.0g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
Preparation of Salts A Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Maintain for 3 days at 25°C to dissolve. Filter sterilize the supernatant.
Salts B:
Composition per 100.0mL:
MnSO 4 ..........................................................................................0.1g
Na 2 MoO 4 ......................................................................................0.1g
Preparation of Salts B Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except salts A solution and salts B solution, to distilled/deionized water and bring volume to 994.5mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 5.0mL of sterile salts A solution and 1.5mL of sterile salts B solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Pseudomonas species.

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