- 定做培养基/定制培养基
- 2020年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
Folic Acid Agar
Folic Acid Agar
培养基配方
Composition per liter:
Noble agar...................................................................................15.0g
K 2 HPO 4 ·3H 2 O ..............................................................................1.2g
Folic acid ......................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
Salts A........................................................................................5.0mL
Salts B........................................................................................1.5mL
Salts A:
Composition per 100.0mL:
MgSO 4 ·7H 2 O................................................................................1.0g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
Preparation of Salts A Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Maintain for 3 days at 25°C to dissolve. Filter sterilize the supernatant.
Salts B:
Composition per 100.0mL:
MnSO 4 ..........................................................................................0.1g
Na 2 MoO 4 ......................................................................................0.1g
Preparation of Salts B Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except salts A solution and salts B solution, to distilled/deionized water and bring volume to 994.5mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 5.0mL of sterile salts A solution and 1.5mL of sterile salts B solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Pseudomonas species.