FX AG Broth

[所属分类:培养基配方] [发布时间:2021-3-8] [发布人: ] [阅读次数:] [返回]

FX AG Broth

培养基配方

Composition per liter:
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Glucose solution ....................................................................100.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
D- Glucose......................................................................................2.0g
Preparation of Glucose Solution: Add  D- glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Cytophaga species, Herpetosiphon species, Saprospira species, and Flexithrix species.