G Medium

[所属分类:培养基配方] [发布时间:2021-3-8] [发布人: ] [阅读次数:] [返回]

培养基配方

Composition per liter:

(NH 4 ) 2 SO 4 .....................................................................................2.0g
Yeast extract..................................................................................2.0g
Glucose .........................................................................................1.0g
K 2 HPO 4 .........................................................................................0.6g
KH 2 PO 4 .........................................................................................0.4g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ...........................................................................................0.08g
MnSO 4 ·H 2 O................................................................................0.05g
CuSO 4 ·5H 2 O..............................................................................5.0mg
ZnSO 4 ·7H 2 O..............................................................................5.0mg
FeSO 4 ·7H 2 O...............................................................................0.5mg
pH 7.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Bacillus cereus.