GBS Agar Base, Islam (Group B Streptococci Agar) (Islam GBS Agar)

[所属分类:培养基配方] [发布时间:2021-3-8] [发布人: ] [阅读次数:] [返回]

培养基配方

Composition per liter:
Proteose peptone.........................................................................23.0g
Agar ............................................................................................10.0g
Na 2 HPO 4 .....................................................................................5.75g
Soluble starch................................................................................5.0g
NaH 2 PO 4 .......................................................................................1.5g
Horse serum, heat inactivated..................................................50.0mL
pH 7.5 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from Oxoid Unipath.
Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile inactivated horse serum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the isolation and detection of group B streptococci (GBS) in clinical specimens. The medium is designed to exploit the ability of most group B streptococci (GBS) to produce orange/red pigmented colonies when incubated under anaerobic conditions. There is a pigment-enhancing effect around a sulphonamide disc, which does not grow; the enhanced pigment effect can be seen over a radius of 10–20mm. Non-group B organisms able to grow on this medium do not produce the orange/red pigment.