GBS Medium, Rapid (Group B Streptococci Medium) (GBS Medium Base)

[所属分类:培养基配方] [发布时间:2021-3-8] [发布人: ] [阅读次数:] [返回]

培养基配方

Starch ......................................................................................... 80.0g
Proteose peptone.........................................................................23.0g
Na 2 HPO 4 .....................................................................................5.75g
NaH 2 PO 4 .......................................................................................1.5g
Horse serum, inactivated..........................................................50.0mL
Antibiotic inhibitor solution....................................................10.0 mL
pH 7.5 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid Unipath and HiMedia.
Antibiotic Inhibitor Solution:
Composition per 10.0mL:
Metronidazole..........................................................................10.0mg
Gentamicin.................................................................................2.0mg
Preparation of Antibiotic Inhibitor Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except horse serum and antibiotic inhibitor solution, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile heat-inactivated horse serum and 10.0mL of sterile antibiotic inhibitor solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Cool to 5°C and hold at that temperature for 12 hr prior to use.
Use: For the rapid isolation and cultivation of group B streptococci from clinical specimens.