GC Agar (ATCC Medium 814)

[所属分类:培养基配方] [发布时间:2021-3-8] [发布人: ] [阅读次数:] [返回]

培养基配方

Composition per 1010.0mL:
GC agar base, 2X...................................................................500.0mL
Hemoglobin solution..............................................................500.0mL
Supplement solution ................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
GC Agar Base, 2X:
Composition per 500.0mL:
Agar ............................................................................................10.0g
Pancreatic digest of casein............................................................7.5g
Peptic digest of animal tissue........................................................7.5g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Cornstarch.....................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Source:  GC agar base is available as a premixed powder from BD Diagnostic Systems. This base may be replaced by GC medium base available from BD Diagnostic Systems.
Preparation of GC Agar Base, 2X: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Hemoglobin Solution:
Composition 500.0mL:
Bovine hemoglobin.....................................................................10.0g
Preparation of Hemoglobin Solution: Add bovine hemoglobin to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Supplement Solution:
Composition per liter:
Glucose .....................................................................................100.0g
L- Cysteine·HCl............................................................................25.9g
L- Glutamine.................................................................................10.0g
L- Cystine .......................................................................................1.1g
Adenine.........................................................................................1.0g
Nicotinamide adenine dinucleotide ............................................0.25g
Vitamin B 12 ...................................................................................0.1g
Thiamine pyrophosphate...............................................................0.1g
Guanine·HCl...............................................................................0.03g
Fe(NO 3 ) 3 ·6H 2 O...........................................................................0.02g
p-Aminobenzoic acid................................................................0.013g
Thiamine·HCl ............................................................................3.0mg
Source: The supplement solution (IsoVitaleX ® enrichment) is available from BD Diagnostic Systems. This enrichment may be replaced by supplement VX from BD Diagnostic Systems.
Preparation of Supplement Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Preparation of Medium: To 500.0mL of sterile GC agar base, aseptically add 500.0mL of sterile hemoglobin solution at 45°–50°C. Mix thoroughly. Aseptically add 10.0mL of sterile supplement solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of fastidious bacteria, especially Neisseria and Haemophilus species. For the cultivation and maintenance of Branhamella catarrhalis, Campylobacter pylori, Eikenella corrodens, Helicobacter pylori, Moraxella nonliquefaciens, Morococcus cerebrosis, Oligella ureolytica, Oligella urethralis, Pasteurella volantium, Proteus mirabilis, and Taylorella equigenitalis.