GC Agar (GC Medium) (ATCC Medium 1351)

[所属分类:培养基配方] [发布时间:2021-3-8] [发布人: ] [阅读次数:] [返回]

培养基配方

Composition per liter:
GC agar base..........................................................................950.0mL
Blood, defibrinated..................................................................50.0mL
pH 7.2 ± 0.2 at 25°C
GC Agar Base:
Composition per liter:
Agar............................................................................................10.0g
Pancreatic digest of casein............................................................7.5g
Peptic digest of animal tissue .......................................................7.5g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Cornstarch.....................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Source:  GC agar base is available as a premixed powder from BD Diagnostic Systems. This base may be replaced by GC medium base available from BD Diagnostic Systems.
Preparation of GC Agar Base: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 75°–80°C.
Preparation of Medium: To 950.0mL of sterile GC agar base aseptically add 50.0mL sterile defibrinated blood with thorough mixing and maintain at 75°–80°C for 15–20 min until the medium is chocolatized. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of fastidious bacteria, especially Neisseria and Haemophilus species. For the cultivation and maintenance of Branhamella catarrhalis, Campylobacter pylori, Eikenella corrodens, Helicobacter pylori, Moraxella nonliquefaciens, Morococcus cerebrosis, Oligella ureolytica, Oligella urethralis, Pasteurella volantium, Proteus mirabilis, and Taylorella equigenitalis.