GC Agar Base with Blood

[所属分类:培养基配方] [发布时间:2021-3-9] [发布人: ] [阅读次数:] [返回]

GC Agar Base with Blood

培养基配方

Composition per liter:
Peptone, special ..........................................................................15.0g
Agar............................................................................................10.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
KH 2 PO 4 .........................................................................................1.0g
Cornstarch.....................................................................................1.0g
Blood, defibrinated..................................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium,without blood, is available as a premixed powder from HiMedia.
Preparation of Medium: Add components, except blood, to distilled/deionized water and bring volume to 950.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 75°–80°C. Add 50.0mL sterile defibrinated blood with thorough mixing and maintain at 75°–80°C for 15–20 min until the medium is chocolatized. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of fastidious bacteria, especially Neisseria and Haemophilus species.