GC Agar Base with Kellogg’s Supplement (ATCC Medium 1674)

[所属分类:培养基配方] [发布时间:2021-3-9] [发布人: ] [阅读次数:] [返回]

GC Agar Base with Kellogg’s Supplement

(ATCC Medium 1674)

培养基配方

Composition per liter:
GC agar base..........................................................................990.0mL
Kellogg’s supplement solution.................................................10.0mL
pH 7.2 ± 0.2 at 25°C
GC Agar Base:
Composition per 990.0mL:
Agar ............................................................................................10.0g
Pancreatic digest of casein............................................................7.5g
Peptic digest of animal tissue........................................................7.5g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Cornstarch.....................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Source:  GC agar base is available as a premixed powder from BD Diagnostic Systems. This base may be replaced by GC medium base available from BD Diagnostic Systems.
Preparation of GC Agar Base: Add components to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Kellogg’s Supplement Solution:
Composition per 100.0mL:
Glucose .......................................................................................40.0g
L- Glutamine...................................................................................0.5g
Fe(NO 3 ) 3 ·6H 2 O...........................................................................0.05g
Cocarboxylase............................................................................2.0mg
Preparation of Kellogg’s Supplement Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: To 990.0mL of sterile GC agar base, aseptically add 10.0mL of sterile Kellogg’s supplement solution warmed to 45°–50°C. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Arsenophonus nasoniae.