GC Medium, New York City Formulation

[所属分类:培养基配方] [发布时间:2021-3-9] [发布人: ] [阅读次数:] [返回]

GC Medium, New York City Formulation

培养基配方

Composition per liter:
GC agar base..........................................................................850.0mL
Horse blood, lysed .................................................................100.0mL
Yeast autolysate supplement....................................................30.0mL
LCAT antibiotic solution .........................................................20.0mL
pH 7.3 ± 0.2 at 25°C
GC Agar Base:
Composition per 850.0mL:
Special peptone...........................................................................15.0g
Agar ............................................................................................10.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Cornstarch.....................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
pH 7.2 ± 0.2 at 25°C
Preparation of GC Agar Base: Add components of GC medium base and the hemoglobin to distilled/deionized water and bring volume to 850.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Horse Blood, Lysed:
Composition per 100.0mL:
Saponin .........................................................................................0.5g
Horse blood, defibrinated ......................................................100.0mL
Preparation of Horse Blood, Lysed: Add saponin to defibrinated horse blood. Mix thoroughly. Allow blood to lyse.
Yeast Autolysate Supplement:
Composition per 30.0mL:
Yeast autolysate...........................................................................10.0g
Glucose .........................................................................................1.0g
NaHCO 3 ......................................................................................0.15g
Preparation of Yeast Autolysate Supplement: Add components to distilled/deionized water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize.
LCAT Antibiotic Solution:
Composition per 20.0mL:
Colistin.......................................................................................6.0mg
Trimethoprim lactate..................................................................5.0mg
Lincomycin................................................................................1.0mg
Amphotericin B..........................................................................1.0mg
Preparation of LCAT Antibiotic Solution: Add components to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: To 850.0mL of cooled sterile GC agar base, aseptically add 100.0mL of sterile lysed horse blood, 30.0mL of sterile yeast autolysate supplement, and 20.0mL of LCAT antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation and cultivation of fastidious microorganisms, especially Neisseria species.