GCII Agar

[所属分类:培养基配方] [发布时间:2021-3-9] [发布人: ] [阅读次数:] [返回]

GCII Agar

培养基配方

Composition per liter:
GCII agar base, 2X................................................................490.0mL
Hemoglobin solution .............................................................490.0mL
Supplement solution ................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
GCII Agar Base, 2X:
Composition per liter:
Agar............................................................................................10.0g
Pancreatic digest of casein............................................................7.5g
Selected meat peptone ..................................................................7.5g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Cornstarch.....................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Source:  GCII agar base is available as a premixed powder from BD Diagnostic Systems.
Preparation of GCII Agar Base, 2X: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Hemoglobin Solution:
Composition per 500.0mL:
Hemoglobin................................................................................10.0g
Preparation of Hemoglobin Solution: Add hemoglobin to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Supplement Solution:
Composition per liter:
Glucose.....................................................................................100.0g
L- Cysteine·HCl............................................................................25.9g
L- Glutamine ................................................................................10.0g
L- Cystine.......................................................................................1.1g
Adenine.........................................................................................1.0g
Nicotinamide adenine dinucleotide ............................................0.25g
Vitamin B 12 ...................................................................................0.1g
Thiamine pyrophosphate ..............................................................0.1g
Guanine·HCl...............................................................................0.03g
Fe(NO 3 ) 3 ·6H 2 O...........................................................................0.02g
p-Aminobenzoic acid................................................................0.013g
Thiamine·HCl............................................................................3.0mg
Preparation of Supplement Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Preparation of Medium: To 490.0mL of sterile GCII agar base, aseptically add 490.0mL of sterile hemoglobin solution at 45°–50°C. Mix thoroughly. Aseptically add 10.0mL of sterile supplement solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophilus species, from clinical specimens.