GCII Agar

[所属分类:培养基配方] [发布时间:2021-3-9] [发布人: ] [阅读次数:] [返回]

GCII Agar

培养基配方

Composition per liter:
GCII agar base.......................................................................950.0mL
Blood, defibrinated..................................................................50.0mL
pH 7.2 ± 0.2 at 25°C
GCII Agar Base with Extra Agar:
Composition per liter:
Agar ............................................................................................12.0g
Pancreatic digest of casein............................................................7.5g
Selected meat peptone ..................................................................7.5g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Cornstarch.....................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Source:  GCII agar base is available as a premixed powder from BD Diagnostic Systems.
Preparation of GCII Agar Base with Extra Agar: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: To 950.0mL of sterile GCII agar base, aseptically add 50.0mL of sterile defibrinated blood with thorough mixing and maintain at 75°–80°C for 15–20 min until the medium is chocolatized. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophilus species, from clinical specimens.