Giolitti-Cantoni Broth (Giolitti-Cantoni Broth Base with Tellurite)

[所属分类:培养基配方] [发布时间:2021-3-11] [发布人: ] [阅读次数:] [返回]

Giolitti-Cantoni Broth

(Giolitti-Cantoni Broth Base with Tellurite)

培养基配方

Composition per 1030.0mL:
Mannitol......................................................................................20.0g
Pancreatic digest of casein..........................................................10.0g
Beef extract...................................................................................5.0g
LiCl...............................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Sodium pyruvate...........................................................................3.0g
Glycine..........................................................................................1.2g
Chapman tellurite solution.........................................................0.3mL
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid Unipath and BD Diagnostic Systems.
Chapman Tellurite Solution:
Composition per 100.0mL:
K 2 TeO 3 ..........................................................................................3.5g
Preparation of Chapman Tellurite Solution: Add K 2 TeO 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Caution: Potassium tellurite is toxic.

Preparation of Medium: Add components, except Chapman tellurite solution, to distilled/deionized water and bring volume to 1.0L. Gently heat and bring to boiling. Mix thoroughly. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool rapidly to 25°C. Immediately prior to use, incubate tubes at 100°C to expel oxygen. Aseptically add 0.3mL of sterile Chapman tellurite solution to each tube. Mix thoroughly. Cool to 25°C.
Use: For the cultivation and enrichment of Staphylococcus aureus from foods.