Gisa Agar

[所属分类:培养基配方] [发布时间:2021-3-11] [发布人: ] [阅读次数:] [返回]

Gisa Agar

培养基配方

Composition per liter:

Agar............................................................................................18.0g
(NH 4 ) 2 SO 4 ....................................................................................5.0g
Calcium glucoisosaccharinate ......................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
NaCl..............................................................................................0.1g
CaCl 2 ·2H 2 O ..................................................................................0.1g
Inositol.......................................................................................2.0mg
KI...............................................................................................1.0mg
H 3 BO 3 ........................................................................................0.5mg
ZnSO 4 ·7H 2 O..............................................................................0.4mg
MnSO 4 ·4H 2 O.............................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
Pyroxidine·HCl..........................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin..................................................................................0.2mg
FeCl 3 ..........................................................................................0.2mg
Na 2 MoO 4 ·4H 2 O.........................................................................0.2mg
CuSO 4 ·5H 2 O............................................................................0.04mg
Folic acid ....................................................................................2.0μg
Biotin..........................................................................................2.0μg
pH 5.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Ancylobacter aquaticus, Pseudomonas species, Xanthobacter autotrophicus, and Xanthobacter species.