Glucose Broth, Buffered

[所属分类:培养基配方] [发布时间:2021-3-12] [发布人: ] [阅读次数:] [返回]

Glucose Broth, Buffered

培养基配方

Composition per liter:
Peptone..........................................................................................5.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .......................................................................................3.68g
Yeast extract..................................................................................2.0g
KH 2 PO 4 .......................................................................................1.32g
Lab Lemco beef extract ................................................................1.0g
Glucose .........................................................................................1.0g
pH 7.2 ± 0.2 at 25°C

Source:  CM1 broth powder, which contains the peptone, NaCl, yeast extract, and Lab Lemco beef extract, is available from Oxoid Unipath. 

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Aerococcus viridans, Carnobacterium alterfunditum, Carnobacterium funditum, Enterococcus durans, Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Lactococcus lactis, Lactococcus plantarum, Streptococcus agalactiae, Streptococcus equismilis, Streptococcus mutans, Streptococcus pyogenes, Streptococcus salivarius, and other Streptococcus species.