Glucose Broth, 9K

[所属分类:培养基配方] [发布时间:2021-3-12] [发布人: ] [阅读次数:] [返回]

Glucose Broth, 9K

培养基配方

Composition per liter:
Glucose .......................................................................................10.0g
(NH 4 ) 2 SO 4 .....................................................................................3.0g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
KCl................................................................................................0.1g
Ca(NO 3 ) 2 .................................................................................0.0125g
FeSO 4 ·7H 2 O.............................................................................0.01mg
Glucose solution ....................................................................100.0mL
pH 3.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose .......................................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 3.5 with H 2 SO 4 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 100.0mL of sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use:  For the cultivation of Thiobacillus acidophilus.