Glucose Cysteine Agar Base with Thiamine and Hemoglobin

[所属分类:培养基配方] [发布时间:2021-3-12] [发布人: ] [阅读次数:] [返回]

Glucose Cysteine Agar Base

with Thiamine and Hemoglobin

培养基配方

Composition per liter:
Glucose.......................................................................................25.0g
Papaic digest of soyabean meal..................................................10.0g
Agar............................................................................................14.0g
NaCl..............................................................................................5.0g
Meat peptone ................................................................................3.0g
Cysteine·HCl.................................................................................1.0g
Thiamine....................................................................................5.0mg
Hemoglobin solution .............................................................500.0mL
pH 6.9 ± 0.2 at 25°C
Source: This medium,without hemoglobin solution, is available as a premixed powder from HiMedia.
Hemoglobin Solution:
Composition 500.0mL:
Bovine hemoglobin.....................................................................10.0g
Preparation of Hemoglobin Solution: Add bovine hemoglobin to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add components, except hemoglobin, to distilled/deionized water and bring volume to 500.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Add 500.0mL sterile hemoglobin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For thecultivation and enumeration of Pasteurella tularensis.