Glucose Yeast Extract Agar

[所属分类:培养基配方] [发布时间:2021-3-15] [发布人: ] [阅读次数:] [返回]

Glucose Yeast Extract Agar

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
Peptic digest of animal tissue........................................................5.0g
Yeast extract..................................................................................5.0g
Glucose .........................................................................................2.0g
KH 2 PO 4 .........................................................................................0.5g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.3g

NaCl............................................................................................0.01g

MnSO 4 ·H 2 O................................................................................0.01g
ZnSO 4 ·7H 2 O..............................................................................1.6mg
CuSO 4 ·5H 2 O..............................................................................1.6mg
CoSO 4 ·7H 2 O..............................................................................1.6mg
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the enumeration and cultivation of lactobacilli in pharmaceutical preparations.