Glucose Yeast Extract Iron Agar (LMG Medium 153)

[所属分类:培养基配方] [发布时间:2021-3-15] [发布人: ] [阅读次数:] [返回]

Glucose Yeast Extract Iron Agar

(LMG Medium 153)

培养基配方

Composition per liter:
Agar............................................................................................15.0g
Glucose.......................................................................................10.0g
Yeast extract..................................................................................5.0g
Iron solution.............................................................................20.0mL
pH 7.1 ± 0.2 at 25°C
Iron Solution:
Composition per 100.0mL:
FeCl 3 ...........................................................................................0.03g
Preparation of Iron Solution: Add FeCl 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except iron solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL sterile iron solution. Mix thoroughly. Aseptically pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Pseudomonas denitrificans. Note: Buffered Nutrient Agar is more commonly used for the culture of P. denitrificans.