Glutamate Medium

[所属分类:培养基配方] [发布时间:2021-3-17] [发布人: ] [阅读次数:] [返回]

Glutamate Medium

培养基配方

Composition per liter:
Solution A..............................................................................500.0mL
Solution B..............................................................................250.0mL
Solution C..............................................................................250.0mL
Solution A:
Composition per 500.0mL:
Mannitol......................................................................................10.0g
K 2 HPO 4 .......................................................................................0.22g
Preparation of Solution A: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Solution B:
Composition per 250.0mL:
MgSO 4 ·7H 2 O................................................................................0.1g
CaCl 2 ·6H 2 O.................................................................................0.08g
FeCl 3 ·6H 2 O.................................................................................0.05g
Preparation of Solution B:  Add components to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Solution C:
Composition per 250.0mL:
Sodium glutamate.........................................................................1.1g
Calcium pantothenate ................................................................0.5mg
Thiamine·HCl ............................................................................0.1mg
Biotin ..........................................................................................0.5μg
Preparation of Solution C: Add components to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Aseptically combine 500.0mL of cooled, sterile solution A, 250.0mL of cooled, sterile solution B, and 250.0mL of sterile solution C. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the isolation of Rhizobium species.