Glutamate Medium (ATCC Medium 820)

[所属分类:培养基配方] [发布时间:2021-3-17] [发布人: ] [阅读次数:] [返回]

Glutamate Medium

(ATCC Medium 820)

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
Sodium glutamate.........................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
KCl................................................................................................0.1g
Glucose solution....................................................................100.0mL
pH 6.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose.......................................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Pseudomonas species.