Glycerol Agar

[所属分类:培养基配方] [发布时间:2021-3-17] [发布人: ] [阅读次数:] [返回]

Glycerol Agar

培养基配方

Composition per 1070.0mL:
Agar............................................................................................15.0g
Peptone .........................................................................................5.0g
Beef extract...................................................................................3.0g
Soil extract....................................................................................1.0L
Glycerol ...................................................................................70.0mL
pH 7.0 ± 0.2 at 25°C
Soil Extract:
Composition per liter:
Soil, air dried ............................................................................. 1.0Kg
Preparation of Soil Extract: Sift soil through a #9 mesh screen. Add to 2.4L of tap water. Mix thoroughly. Autoclave for 60 min at 15 psi pressure–121°C. Cool to 25°C. Filter through Whatman #1 filter paper. Bring volume to 1.0L with tap water.

Preparation of Medium: Combine components. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation and cultivation of Nocardia species and Rhodococcus species.