Glycerol Agar

[所属分类:培养基配方] [发布时间:2021-3-17] [发布人: ] [阅读次数:] [返回]

Glycerol Agar

 培养基配方

Composition per liter:
Beef heart, solids from infusion................................................250.0g
Glycerol ......................................................................................60.0g
Agar............................................................................................15.0g
Pancreatic digest of gelatin...........................................................5.0g
Tryptose........................................................................................5.0g
Beef extract...................................................................................3.0g
NaCl..............................................................................................2.5g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use:  For the cultivation and maintenance of Bacillus subtilis, Enterococcus faecalis, Erwinia chrysanthemi, Gordona rubropertinctus, Mycobacterium species, Nocardia brevicatena, Rhodococcus equi, and Rhodococcus rhodochrous.