Glycerol Agar

[所属分类:培养基配方] [发布时间:2021-3-17] [发布人: ] [阅读次数:] [返回]

Glycerol Agar

培养基配方

Composition per liter:
Beef heart, infusion from..........................................................300.0g
Glycerol ......................................................................................60.0g
Agar ............................................................................................17.5g
Tryptose ........................................................................................7.0g
NaCl..............................................................................................3.0g
Peptone..........................................................................................2.5g
NaCl..............................................................................................2.5g
Yeast extract..................................................................................1.0g
Beef extract...................................................................................0.5g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of fastidious bacteria.