Glycerol Soil Agar

[所属分类:培养基配方] [发布时间:2021-3-18] [发布人: ] [阅读次数:] [返回]

Glycerol Soil Agar

培养基配方

Composition per liter:
Glycerol ......................................................................................20.0g
Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
Beef extract...................................................................................3.0g
Soil extract.............................................................................150.0mL
pH 7.0 ± 0.2 at 25°C
Soil Extract:
Composition per liter:
Soil............................................................................................400.0g
Preparation of Soil Extract:  Air dry garden soil and pass through a sieve. Weigh out 400.0g and add to 960.0mL of tap water. Autoclave for 60 min at 15 psi pressure–121°C. Cool to room temperature and allow soil to settle out. Decant supernatant solution. Filter through paper. Distribute into flasks in 200.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Actinomadura madurae, Amycolata autotrophica, Amycolata saturnea, Gordona bronchialis, Gordona terrae, Mycobacterium species, Nocardia species, and Tsukamurella paurometabolum.