Glycine Cycloheximide Phenol Red Agar

[所属分类:培养基配方] [发布时间:2021-3-18] [发布人: ] [阅读次数:] [返回]

Glycine Cycloheximide Phenol Red Agar

培养基配方

Composition per liter:
Solution B..............................................................................800.0mL
Solution A..............................................................................200.0mL
Solution A:
Composition per 200.0mL:
Glycine........................................................................................10.0g
(NH 4 ) 2 SO 4 .....................................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
NaCl..............................................................................................0.1g
CaCl 2 ·2H 2 O...................................................................................0.1g
DL- Methionine.............................................................................0.02g
DL- Tryptophan.............................................................................0.02g
L- Histidine·HCl...........................................................................0.01g
Inositol.......................................................................................2.0mg
H 3 BO 3 ........................................................................................0.5mg
ZnSO 4 ·7H 2 O..............................................................................0.4mg
MnSO 4 ·4H 2 O.............................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
Pyroxidine·HCl..........................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin ..................................................................................0.2mg
FeCl 3 ..........................................................................................0.2mg
Na 2 MoO 4 ·4H 2 O .........................................................................0.2mg

KI...............................................................................................0.1mg

CuSO 4 ·5H 2 O............................................................................0.04mg
Folic acid ....................................................................................2.0μg
Biotin..........................................................................................2.0μg
Cycloheximide solution.............................................................1.6mL
Preparation of Solution A: Add components to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize.
Cycloheximide Solution:
Composition per 100.0mL:
Cycloheximide..............................................................................0.1g
Preparation of Cycloheximide Solution: Add cylcohexamide to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.
Solution B:
Composition per 800.0mL:
Agar............................................................................................20.0g
Phenol Red solution.................................................................30.0mL
Preparation of Solution B: Add components to distilled/deionized water and bring volume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Coolto 50°–55°C.
Phenol Red Solution:
Composition per 100.0mL:
Phenol Red....................................................................................0.5g
Preparation of Phenol Red Solution: Add Phenol Red to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: To 800.0mL of sterile solution B, aseptically add 200.0mL of sterile solution A at 55°C. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective cultivation and differentiation of fungi from clinical and nonclinical specimens. Cryptococcus neoformans turns the medium bright red.