Glycocholate Mineral Medium

[所属分类:培养基配方] [发布时间:2021-3-18] [发布人: ] [阅读次数:] [返回]

Glycocholate Mineral Medium

培养基配方

Composition per liter:
Agar............................................................................................15.0g
K 2 HPO 4 .........................................................................................3.5g
(NH 4 ) 2 SO 4 ....................................................................................2.0g
Sodium glycocholate ....................................................................2.0g
KH 2 PO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.1g
Yeast extract..................................................................................0.1g
CaCl 2 ·2H 2 O ................................................................................0.01g
FeSO 4 ·7H 2 O...............................................................................0.5mg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Pseudomonas putida and Pseudomonas species.