GPVA Medium

[所属分类:培养基配方] [发布时间:2021-3-19] [发布人: ] [阅读次数:] [返回]

GPVA Medium

培养基配方

Composition per liter:
Agar............................................................................................15.0g
Yeast extract................................................................................10.0g
ACES buffer (2-[(2-amino-2-oxoethyl)-
amino]-ethane sulfonic acid)................................................10.0g
Glycine..........................................................................................3.0g
Charcoal, activated .......................................................................2.0g
α-Ketoglutarate.............................................................................1.0g
Fe 4 (P 2 O 7 ) 3 ·9H 2 O.........................................................................0.25g
Antibiotic inhibitor solution ....................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Antibiotic Inhibitor Solution:
Composition per 10.0mL:
Anisomycin.................................................................................0.08g
Vancomycin...............................................................................5.0mg
Polymyxin B........................................................................ 100,000U
Preparation of Antibiotic Inhibitor Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except antibiotic inhibitor solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Adjust pH to 6.9. Aseptically add 10.0mL of sterile antibiotic inhibitor solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Legionella species from environmental waters.