Grahamella Medium

[所属分类:培养基配方] [发布时间:2021-3-19] [发布人: ] [阅读次数:] [返回]

Grahamella Medium

培养基配方

Composition per liter:
Agar............................................................................................20.0g
Saline (0.9% solution) ...........................................................800.0mL
Rabbit serum, filter sterilized ..................................................90.0mL
Rabbit hemoglobin (2% solution)............................................45.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except rabbit serum and rabbit hemoglobin, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into small tubes in 2.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°C. Aseptically add 0.2mL of sterile rabbit serum and 0.1mL of rabbit hemoglobin solution to each tube. Mix thoroughly. Autoclave for 30 min with a mixture of steam and air at 0 psi pressure–56°C.
Use: For the cultivation of Grahamella species.